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Fig. 3 | BMC Biotechnology

Fig. 3

From: Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

Fig. 3

Positive feedback of Mxr1 increased the transcriptional efficiency of PAOX1 and broke the Mxr1 titration effect. mRNA was extracted from cells cultured in different carbon sources for 3 h. a GFP. b MXR1. mRNA levels in each sample were normalized to 18S rRNA. The relative expression level for each gene was normalized to the control grown under the carbon-free condition. The error bars represent the standard deviation of three biological replicates. Two-way ANOVA and the Tukey test were used to determine significance. Groups with different letters are significantly different. c AOX activity in different cells in response to different carbon sources

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