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Fig. 4 | BMC Biotechnology

Fig. 4

From: Establishment of TP53-knockout canine cells using optimized CRIPSR/Cas9 vector system for canine cancer research

Fig. 4

Non-tumorigenic potential of CRISPR/Cas9-mediated Tp53KO#30 canine fibroblasts in vitro. a Immunoblot data showing HRAS, total AKT (tAKT), phosphorylated AKT (pAKT), tERK1/2, and pERK1/2 protein levels in TP53KO#30, TP53KO#39, and their corresponding HRASV12-transduced cells. β-Actin was used as the loading control. b Representative images showing the cellular morphologies of TP53KO#30, TP53KO#39, and their corresponding HRASV12-transduced cells. c Representative images showing the generated colonies of TP53KO#30, TP53KO#39, and their corresponding HRASV12-transduced cells grown in semi-solid agar cultures for 32 days. d Quantitative data of colony numbers in semi-solid agar cultures. e Proliferation rates of TP53KO#30, TP53KO#39, and their corresponding HRASV12-transduced cells

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