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Table 1 Bacterial strains and plasmids used in this study

From: An auto-inducible phosphate-controlled expression system of Bacillus licheniformis

Strains or plasmids Relevant genotype Reference
Strains
E. coli DH10B F−, mrcA, Δ(mrr-hsdRMS-mrcBC), Φ80dlacZ, ΔM15, ΔlacX74, deoR, ecA1,endA1,araD139, Δ(ara,leu)7697, galU, galK, λ−, nrspL, nupG Gibco BRL
B. licheniformis MW3 ΔhsdR1, ΔhsdR2 [16]
B. licheniformis TH3 ΔhsdR1, ΔhsdR2, pKUC3 This study
B. licheniformis TH4 ΔhsdR1, ΔhsdR2, pKUC4 This study
Plasmids
 pKUC shuttle vector based on pUC18 and pKTH290 [19]
 pKUC3 pKUC containing the PphyL′-′amyE fusion This study
 pKUC4 pKUC containing the PphyL′-′xynA fusion This study