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Fig. 3 | BMC Biotechnology

Fig. 3

From: An auto-inducible phosphate-controlled expression system of Bacillus licheniformis

Fig. 3

The expression of the recombinant amylase and xylanase driven by the phyL promoter (n = 3 independent cultivations) of the B. licheniformis strains TH3 (a) and TH4 (b) without and with the addition of sodium phytate. Sodium phytate was added at an OD500 nm of 1.0 (after 8 h cultivation). Lines indicate cell growth, while bars indicate enzyme activity. Growth: Open symbols TH3, filled symbols TH4; triangles without the addition of sodium phytate, circles with 0.5 mM sodium phytate, squares with 5 mM sodium phytate. Enzyme activity: white bars without the addition of sodium phytate, grey bars with 0.5 mM sodium phytate, black bars with 5 mM sodium phytate. SDS-PAGE separation of extracellular proteins in 20 μL of culture supernatant of strain TH4 without addition of sodium phytate (c) and with 0.5 mM sodium phytate (d). Sodium phytate was added at an OD 500 nm of 1.0 (after 8 h cultivation). Protein bands are indicated by arrows

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