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Fig. 2 | BMC Biotechnology

Fig. 2

From: An auto-inducible phosphate-controlled expression system of Bacillus licheniformis

Fig. 2

The phyL promoter-dependend expression of the recombinant amylase and xylanase encoding genes of the B. licheniformis strains TH3 (a) and TH4 (b) under phosphate limited growth conditions (n = 3 independent cultivations). Lines indicate cell growth, while bars indicate enzyme activity. Growth: triangles TH3, squares TH4. Enzyme activity: grey bars amylase, black bars xylanase. c and d are SDS-PAGE separations of extracellular proteins in B. licheniformis strains TH3 and TH4, respectively. Each sample contained 20 μL of culture medium after removal of the cells by centrifugation. Protein bands are indicated by arrows

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