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Fig. 2 | BMC Biotechnology

Fig. 2

From: Expression of Rift Valley fever virus N-protein in Nicotiana benthamiana for use as a diagnostic antigen

Fig. 2

Transient expression time trial of N-protein in N. benthamiana by Agrobacterium-mediated transfer. Infiltration at OD600 of 0.25, 0.5, and 1. Lanes 1–4 denote the number of days post infiltration (dpi). Lane “-“, − negative control represented by extract from leaves infiltrated with pEAQ-HT vector lacking any gene of interest. Lane M contains PageRuler™ Prestained protein ladder (Thermo Scientific, MA, USA). The protein was detected with 1:5000 anti-N primary antibody and 1:5000 anti-rabbit secondary antibody

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