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Fig. 1 | BMC Biotechnology

Fig. 1

From: Expression of Rift Valley fever virus N-protein in Nicotiana benthamiana for use as a diagnostic antigen

Fig. 1

Schematic presentation and confirmation of pEAQ-HT-his-N. a pEAQ-HT vector components; RB and LB: right and left borders for T-DNA integration, 35S promoter from Cauliflower mosaic virus (CaMV), 5’UTR: modified 5’ UTR from CPMV RNA-2, 3’UTR from CPMV RNA-2, NosT: nopaline synthase terminator, P19: suppressor of gene silencing from TBSV, 35S terminator from CaMV, nptII: kanamycin resistance gene, OriV: pRK2 origin of replication, TrfA: replication essential locus and ColEI; the pBR322 E. coli origin of replication [48]. b Confirmation of pEAQ-HT-his-N (9947 and 773 bp) by restriction enzyme digest mapping with NruI and StuI, 1 kb O’GreenGene ruler (M) was used as a DNA ladder. DNA was resolved on 0.8% (w/v) TBE agarose gel

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BMC Biotechnology

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