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Table 1 Haemagglutination test of rFIP-gap1, rFIP-gap2, rLZ-8 and rFIP-gsi

From: Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganoderma Species

Sources of red blood cells PBS PHA rFIP-gap1 rFIP-gap2 rLZ-8 rFIP-gsi
Human ++ + ++
  ++ + ++
Sheep ++ ++ ++ ++
  ++ + ++ ++
Mouse ++ ++ ++ ++
  ++ ++ ++ +
  1. Note: Haemagglutinating examination of various Ganoderma FIPs including rFIP-gap1, rFIP-gap2, rLZ-8 and rFIP-gsi (all final concentration at 5 μg mL− 1) towards human, sheep and mouse red blood cells. PBS and PHA (5 μg mL− 1) served as negative and positive controls. (+) indicates positive reaction and (−) indicates negative reaction. (++) and (+) denotes 75 and 50% of cells underwent haemagglutination, respectively. All results were from biological duplicate tests