Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganoderma Species

Table 1 Haemagglutination test of rFIP-gap1, rFIP-gap2, rLZ-8 and rFIP-gsi

Sources of red blood cells	PBS	PHA	rFIP-gap1	rFIP-gap2	rLZ-8	rFIP-gsi
Human	–	++	+	–	–	++
	–	++	+	–	–	++
Sheep	–	++	++	–	++	++
	–	++	+	–	++	++
Mouse	–	++	++	–	++	++
	–	++	++	–	++	+

Note: Haemagglutinating examination of various Ganoderma FIPs including rFIP-gap1, rFIP-gap2, rLZ-8 and rFIP-gsi (all final concentration at 5 μg mL^− 1) towards human, sheep and mouse red blood cells. PBS and PHA (5 μg mL^− 1) served as negative and positive controls. (+) indicates positive reaction and (−) indicates negative reaction. (++) and (+) denotes 75 and 50% of cells underwent haemagglutination, respectively. All results were from biological duplicate tests

ISSN: 1472-6750