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Table 1 Percentage extraction of sugars from SESC-treated cedar slurry at different pH values in different saccharification stages (1st, 2nd, 3rd and 4th)

From: Simultaneous enzymatic saccharification and comminution for the valorization of lignocellulosic biomass toward natural products

pH (−) Glucose extraction (%) Cellobiose extraction (%) Total sugar
1st 2nd 3rd 4th Total 1st 2nd 3rd 4th Total
5 33.6 16.7 6.3 1.2 57.8 10.7 0 0 0 10.7 67.3
5.5 35.5 27.7 10.5 2.9 76.6 3.2 1.2 0 0 4.4 81.0
6 41.6 21.6 10.0 3.5 76.7 6.0 1.1 0 0 7.1 83.8
6.5 12.8 34.7 14.8 4.8 67.1 8.1 5.7 0 0 13.8 80.9
7 3.3 21.5 14.9 11.0 50.7 6.6 12.0 10.5 1.1 30.2 80.9
  1. Jet-milled slurry was subjected to two-stage bead milling at 50 °C using 0.5- and 0.1-mmΦ zirconia beads, respectively. An enzyme cocktail of 0.2 mL/g wood biomass (30 FPU/g glucan) was added in each saccharification stage (see Experimental Methods). Phosphate buffer (100 mM) was used for pH control. All values are the average of two analysis runs