Simultaneous enzymatic saccharification and comminution for the valorization of lignocellulosic biomass toward natural products

Table 1 Percentage extraction of sugars from SESC-treated cedar slurry at different pH values in different saccharification stages (1st, 2nd, 3rd and 4th)

pH (−)	Glucose extraction (%)					Cellobiose extraction (%)					Total sugar
pH (−)	1st	2nd	3rd	4th	Total	1st	2nd	3rd	4th	Total	Total sugar
5	33.6	16.7	6.3	1.2	57.8	10.7	0	0	0	10.7	67.3
5.5	35.5	27.7	10.5	2.9	76.6	3.2	1.2	0	0	4.4	81.0
6	41.6	21.6	10.0	3.5	76.7	6.0	1.1	0	0	7.1	83.8
6.5	12.8	34.7	14.8	4.8	67.1	8.1	5.7	0	0	13.8	80.9
7	3.3	21.5	14.9	11.0	50.7	6.6	12.0	10.5	1.1	30.2	80.9

Jet-milled slurry was subjected to two-stage bead milling at 50 °C using 0.5- and 0.1-mmΦ zirconia beads, respectively. An enzyme cocktail of 0.2 mL/g wood biomass (30 FPU/g glucan) was added in each saccharification stage (see Experimental Methods). Phosphate buffer (100 mM) was used for pH control. All values are the average of two analysis runs

ISSN: 1472-6750