Fig. 7From: Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virusNS1 of ZIKV refolded at HHP and pH of 11.0 is antigenic. a NS1 was used as a solid phase antigen in ELISA assays employing control sera obtained from patients that had been previously infected with ZIKV (black bars) or not (white bars). Values are expressed as mean ± error of antigen-specific IgG antibody titers; b Evaluation of the preservation of conformational epitopes of the ZIKV NS1. NS1 obtained at HHP and pH 11.0 was submitted to denaturation (heating at 100 °C for 10 min followed by heat shock at 0 °C) or not and analyzed by ELISA for reactivity with a serum from a patient previously infected with ZIKV. The values obtained are expressed as mean ± error of the absorbance obtained in the assay. * p < 0.05; ** p < 0.01; *** p < 0.001 (Two-way ANOVA with Bonferroni test). The IB compression was performed in 2.4 kbar / 0.4 kbar. Control: NS1 obtained from the same clone used in this study and refolded using traditional protocol at atmospheric pressureBack to article page