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Fig. 7 | BMC Biotechnology

Fig. 7

From: Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus

Fig. 7

NS1 of ZIKV refolded at HHP and pH of 11.0 is antigenic. a NS1 was used as a solid phase antigen in ELISA assays employing control sera obtained from patients that had been previously infected with ZIKV (black bars) or not (white bars). Values are expressed as mean ± error of antigen-specific IgG antibody titers; b Evaluation of the preservation of conformational epitopes of the ZIKV NS1. NS1 obtained at HHP and pH 11.0 was submitted to denaturation (heating at 100 °C for 10 min followed by heat shock at 0 °C) or not and analyzed by ELISA for reactivity with a serum from a patient previously infected with ZIKV. The values obtained are expressed as mean ± error of the absorbance obtained in the assay. * p < 0.05; ** p < 0.01; *** p < 0.001 (Two-way ANOVA with Bonferroni test). The IB compression was performed in 2.4 kbar / 0.4 kbar. Control: NS1 obtained from the same clone used in this study and refolded using traditional protocol at atmospheric pressure

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