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Fig. 6 | BMC Biotechnology

Fig. 6

From: Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus

Fig. 6

Conformational analysis of refolded NS1. a Supernatants from suspensions of NS1-IBs refolded by the application of HHP (2.4 kbar / 0.4 kbar) were dialyzed and analyzed by SDS-PAGE. Condition 1: pH 11.0 + DTT (2 mM); condition 2: pH 11.0 + Arg (0.4 M), condition 3: pH 11.0 + Arg + GSH (1 mM) + GSSG (0.1 mM); condition 4: pH 11.5 + Arg; condition 5: pH 11.5 + Arg + GSH/GSSG. Sample IB: The suspension was homogenized for application in the SDS-PAGE. All the samples were heated at 95 °C in the presence of DTT (100 mM) before application in SDS-PAGE; b Far-UV CD spectra of NS1 (2 μM recorded in 0.2 cm path length quartz cell) refolded at pH 11 in the presence of Arg

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