Fig. 5

NS1 solubilized by application of HHP at a pH of 11.5 forms oligomers after dialysis at a pH of 8.5. ZIKV NS1-IB suspensions were subjected to 2.4 kbar/0.4 kbar at a pH of 11.5 and dialyzed against 50 mM Tris HCl at a pH of 8.5. A volume of 500 μL of the supernatant of the suspensions was applied to a Superdex 200 10/300 column (GE Biosciences). The elution buffer was TrisHCl 50 mM at a pH of 8.5