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Fig. 3 | BMC Biotechnology

Fig. 3

From: Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus

Fig. 3

Application of HHP at alkaline pH solubilizes NS1-IBs and the presence of Arg helps to dissociate oligomers. SEC from supernatants of ZIKV NS1-IB suspensions subjected to 2.4 kbar/0.4 kbar. A volume of 500 μL of the supernatants of the suspensions subjected to HHP was applied to a Superdex 200 10/300 column (GE Biosciences). The elution buffer was 50 mM CAPS at a pH of 11.0

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