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Fig. 3 | BMC Biotechnology

Fig. 3

From: High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension

Fig. 3

Conformational characterization of purified Dicer. (a) SEC-MALS/RI analysis of purified WT Dicer stored in sucrose/DDM-free storage buffer. The relative signal of light scattering (red), refractive index (blue), and UV absorbance at 280 nm (black) are represented by solid lines. The molar mass distribution is shown with green dots. A small amount of aggregated protein can be seen mainly from the light scattering signal. Peak integration of the UV absorbance trace shows that the main peak contains ≥98% of the eluted protein. The molar mass calculated from MALS analysis was normalized against BSA to give an average molecular weight of 224 ± 2 kDa with a polydispersity index of 1.02 ± 0.01 over the entire eluted peak. (b) Negative stain TEM. Left panel Typical grid imaging of negatively-stained WT Dicer. Right panels Main 2D class averages of WT Dicer

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