Fig. 1
From: High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension
Transfection of small suspension cultures with Dicer-expressing plasmid. 293-6E cells were transfected with PEI:pTT5-DNA complexes at a 2:1 mass ratio and monitored after transfection. (a) Transfection efficiency at 48 hpt. A total of 36 ± 7% of the cells are expressing GFP (n = 4). (b) Cell density and viability (n = 4). (c) Expression of cytoplasmic Dicer. Dicer quantification was derived from Western blot analysis using a standard curve of pure recombinant Dicer (n = 4). Transfections in (b) and (c) were performed in 20-mL cultures grown in 125-mL Erlenmeyer flasks and the results are means ± standard deviation values of replicates (n) from independent transfections experiments