Skip to main content

Table 1 Primer list for overlapping PCR to construct PRRSV-N-Rluc and nsp1α- Rluc plasmids

From: Development of luciferase-linked antibody capture assay based on luciferase immunoprecipitation systems for antibody detection of porcine reproductive and respiratory syndrome virus

 

Forward primer

Reverse primer

SD16 N

CGGAATTCATGCCAAATAACAACGGC

CGGTCCGCTACCGGAGCCGCTTGCTG

AGGGTGATGCTGT

HuN4 nsp1α

CAGAATTCATGTCTGGGATACTTG

CGGTCCGCTACCGGAGCCGCTCATAGC

ACACTCAAAAGG

Renilla luciferase

TCCGGTAGCGGACCGGTCGCCACCCTTCCAAGGTGTAC

CCGCGGCCGCTTACTGCTCGTTCTTCAG

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us