Fig. 5
Cell viability on PCL-collagen I-nanoscaffolds. a BMSC/Mb and ADSC/Mb were seeded at different densities (1 × 105, 2 × 105, 3 × 105) on PCL-collagen I-nanoscaffolds and were allowed to proliferate for different time periods: 3 days (d), 7 d, or 14 d. Cell viability was determined by WST 8-assay. Absorbance at a wave length of 450 nm is expressed as mean ± standard deviation. At 14 days, ADSC showed a significantly higher cell viability for 2 × 105 and 3 × 105 cells in comparison to BMSC/Mb. b BMSC/Mb or ADSC/Mb were seeded at a density of 3 × 105 cells on PCL-collagen I-nanoscaffolds. Myogenic differentiation was induced by standard differentiation medium (control) or group 3 serum-free medium. WST 8-assay was repeated after 14 and 28 days of differentiation. Results showed decreased cell viability after serum free differentiation. ADSC/Mb showed higher cell viability compared to BMSC/Mb for all groups. Statistical differences were tested with repeated measures ANOVA for comparison between paired variables and Tukey’s multiple comparisons test as posthoc test at different time points. Pairwise comparison between BMSC/Mb and ADSC/Mb was done using unpaired t-test (n = 3). Level of significance was * p ≤ 0.05