Fig. 4

CK activity of ADSC/Mb and BMSC/Mb after (serum-free) myogenic differentiation. CK activity was measured as extinction during minute 2–6 of reaction time. Cells were allowed to differentiate for three (d3) and 7 days (d7) in serum-free media group 1, 2, 3, and standard myogenic differentiation medium (ctrl.) after 2 days of proliferation (d0). Values are presented as mean ± standard deviation. In BMSC/Mb, ctrl. Led to higher CK activity compared to all serum-free media. Highest CK activity was seen for group 3 of all serum-free media after 7 days of myogenic differentiation. Statistical differences were tested with one-way ANOVA and Bonferroni’s correction for multiple comparisons (n = 3). Levels of significance were * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001