Fig. 1
From: Cyclodextrin enhanced the soluble expression of Bacillus clarkii γ-CGTase in Escherichia coli
Effect of chemical chaperone candidates on γ-CGTase expression in E. coli. a Comparison of soluble γ-CGTase activity and cell growth in shake-flask cultures supplemented with different chemical chaperone candidates. Bars represent extracellular γ-CGTase activity (black), soluble periplasmic γ-CGTase activity (light gray), and DCW (gray). CD, cyclodextrin; DCW, dry cell weight. The results were obtained from three independent experiments. b and c SDS-PAGE analysis of inclusion bodies (b) and soluble γ-CGTases (c) obtained from shake-flask cultures supplemented with different chemical chaperone candidates. The lanes contain: lane 1, control (TB medium with 0.5%(w/v) glycine); lane 2, 1 mM Ca2+; lane 3, 5 mM α-cyclodextrin; lane 4, 5 mM β-cyclodextrin; lane 5, 5 mM γ-cyclodextrin; lane M, molecular mass markers; lane 6, 20 mM sorbitol; lane 7, 20 mM betaine; and lane 8, 20 mM proline. The band corresponding to γ-CGTase is seen near the 66.2 kDa mass marker