Fig. 4

Phosphodegron mutant AAV2 TM displays novel tropism. a Confocal microscopic analysis of testis cryosections stained with lipophilic Nile red and immunostained for GFP. Nile red positive Leydig cells (yellow arrowheads) are not transduced by AAV2 TM. Scale bar 50 and 100 μm, for low and high magnification images, respectively. b Wheat germ agglutinin staining (WGA) staining combined with GFP immunostaining. Pattern of cytoplasmic GFP (yellow arrowheads) indicate transduction of Sertoli cells; WGA high spermatogonial derivatives are GFP negative. Scale bar 50 μm