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Fig. 2 | BMC Biotechnology

Fig. 2

From: Infectivity of adeno-associated virus serotypes in mouse testis

Fig. 2

Infection at higher titre does not alter distribution. Higher titre of AAV2 and AAV5 at 1 X 1010 viral genomes (vgs) were injected per testis and analysed 1 month post-injection, n = 3 animals. a Wholemount of dissected testis showing distribution of live GFP. b Cryosections immunostained with anti-GFP antibody to detect AAV2 transduced cells. Note the distribution of transduced cells outside the seminiferous tubules; SSCs marked by Sal4 are not transduced (Yellow arrowheads). c Graph showing increase in transduction efficiency with higher titre. The number GFP+ cells / 0.58 mm2 (mean ± standard deviation; 52.78 ± 16.45; *p < 0.01; n = 3 animals). d Cryosections immunostained with anti-GFP antibody to detect AAV5 transduced cells. Rare transduced cells were observed with AAV5 injected at 1 X 1010 vgs per testis. Scale bar 50 and 100 μm, for low and high magnification images, respectively

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