Fig. 1

AAV serotypes tested primarily target Leydig cells. a Schematic of the experiment. Direct testicular injection of EGFP encoding viral suspension into the intertubular space in 4 weeks old C57/BL6:DBA2 F1 hybrid males. Viral particles were injected in the right testis of each animal and the left testis served as uninjected control; n = 3 animals. b Illustration of a mouse testis cross-section. c Wholemount of dissected testis showing distribution of live GFP from infected cells. d Combined immunostaining for GFP and Nile red stain (white arrowheads) reveal transduction of Leydig cells by AAV2 and AAV9. e Cryosections immunostained with endothelial marker CD31. Yellow arrowheads highlight absence of GFP and CD31 co-expression. Scale bar 50 and 100 μm, for low and high magnification images, respectively