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Table 3 Experimental design of two-stage temperature control

From: Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11

Scheme Stage Ι Stage II DCW (g L−1) HSAF production (mg L− 1)
Control 0–6 h:26 °C 6–58 h:26 °C 4.35 ± 0.32 400.49 ± 16.41
1 0–6 h:29 °C 6–58 h:26 °C 4.28 ± 0.28 403.45 ± 15.24
2 0–6 h:32 °C 6–58 h:26 °C 4.42 ± 0.24 407.43 ± 13.24
3 0–6 h:35 °C 6–58 h:26 °C 4.15 ± 0.27 376.52 ± 14.74
4 0–6 h:38 °C 6–58 h:26 °C 3.83 ± 0.24 312.21 ± 14.21
5 0–12 h:29 °C 12–58 h:26 °C 4.53 ± 0.26 412.99 ± 16.41
6 0–12 h:32 °C 12–58 h:26 °C 4.43 ± 0.27 440.26 ± 16.14
7 0–12 h:35 °C 12–58 h:26 °C 4.18 ± 0.28 252.03 ± 15.20
8 0–12 h:38 °C 12–58 h:26 °C 3.20 ± 0.24 225.34 ± 13.27
9 0–18 h:29 °C 18–58 h:26 °C 3.62 ± 0.27 266.00 ± 14.65
10 0–18 h:32 °C 18–58 h:26 °C 4.24 ± 0.25 191.07 ± 14.45
11 0–18 h:35 °C 18–58 h:26 °C 3.39 ± 0.22 146.88 ± 13.24
12 0–18 h:38 °C 18–58 h:26 °C 2.33 ± 0.20 164.30 ± 13.18