Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11

Table 3 Experimental design of two-stage temperature control

Scheme	Stage Ι	Stage II	DCW (g L⁻¹)	HSAF production (mg L^− 1)
Control	0–6 h:26 °C	6–58 h:26 °C	4.35 ± 0.32	400.49 ± 16.41
1	0–6 h:29 °C	6–58 h:26 °C	4.28 ± 0.28	403.45 ± 15.24
2	0–6 h:32 °C	6–58 h:26 °C	4.42 ± 0.24	407.43 ± 13.24
3	0–6 h:35 °C	6–58 h:26 °C	4.15 ± 0.27	376.52 ± 14.74
4	0–6 h:38 °C	6–58 h:26 °C	3.83 ± 0.24	312.21 ± 14.21
5	0–12 h:29 °C	12–58 h:26 °C	4.53 ± 0.26	412.99 ± 16.41
6	0–12 h:32 °C	12–58 h:26 °C	4.43 ± 0.27	440.26 ± 16.14
7	0–12 h:35 °C	12–58 h:26 °C	4.18 ± 0.28	252.03 ± 15.20
8	0–12 h:38 °C	12–58 h:26 °C	3.20 ± 0.24	225.34 ± 13.27
9	0–18 h:29 °C	18–58 h:26 °C	3.62 ± 0.27	266.00 ± 14.65
10	0–18 h:32 °C	18–58 h:26 °C	4.24 ± 0.25	191.07 ± 14.45
11	0–18 h:35 °C	18–58 h:26 °C	3.39 ± 0.22	146.88 ± 13.24
12	0–18 h:38 °C	18–58 h:26 °C	2.33 ± 0.20	164.30 ± 13.18

ISSN: 1472-6750