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Table 2 Design and results of the path of steepest ascent experiments

From: Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11

Run Medium capacity
X2 (%)
Culture temperature
X4 (°C)
Fermentation time
X6 (h)
HSAF production (mg L−1)
1 16% 20 44 238.33 ± 14.65
2 18% 24 49 286.47 ± 13.85
3 20% 28 54 376.08 ± 15.43
4 22% 32 59 237.48 ± 14.72
5 24% 36 64 28.38 ± 14.32