Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11

Table 2 Design and results of the path of steepest ascent experiments

Run	Medium capacity X₂ (%)	Culture temperature X₄ (°C)	Fermentation time X₆ (h)	HSAF production (mg L⁻¹)
1	16%	20	44	238.33 ± 14.65
2	18%	24	49	286.47 ± 13.85
3	20%	28	54	376.08 ± 15.43
4	22%	32	59	237.48 ± 14.72
5	24%	36	64	28.38 ± 14.32

ISSN: 1472-6750