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Table 3 Segregation analysis of GFP expression in T1 and T2 progeny of transgenic plants of domesticated einkorn (T. monococcum L.)

From: Genetic transformation of einkorn (Triticum monococcum L. ssp. monococcum L.), a diploid cultivated wheat species

T0 parent plant (T1 progeny plant) Number of plants tested Observed GFP segregation ratio (positive:negative) χ2 value for the expected segregation ratio (positive:negative)
1:1 3:1 15:1
T1 plants
Mn-1 13 8:5 0.69a 1.26a 23.02
Mn-2 179 125:47 35.37 0.50a 130.39
Mn-4 Sterile plant
Mn-7 30 19:11 2.13a 2.18a 47.37
T2 plants
Mn-1(1) 5 3:2 0.20a 0.6a 9.72
Mn-1(2) 6 6:0 Homozygous plants
Mn-2(3) 15 15:0 Homozygous plants
Mn-2(4) 47 36:11 13.30 0.06a 23.60
Mn-2(7) 34 24:10 5.77 0.35a 31.13
Mn-2(10) 18 18:0 Homozygous plants
Mn-7(2) 12 6:6 0.00a 4.00 39.2
Mn-7(3) 33 16:17 0.03a 12.38 115.40
Mn-7(4) 18 10:8 0.22a 3.63a 44.82
  1. a if χ2 value is above 3.84 (P > 0.05) the observed segregation ratio is not significantly different from the expected ratio