Genetic transformation of einkorn (Triticum monococcum L. ssp. monococcum L.), a diploid cultivated wheat species

Table 3 Segregation analysis of GFP expression in T₁ and T₂ progeny of transgenic plants of domesticated einkorn (T. monococcum L.)

T₀ parent plant (T₁ progeny plant)	Number of plants tested	Observed GFP segregation ratio (positive:negative)	χ² value for the expected segregation ratio (positive:negative)
T₀ parent plant (T₁ progeny plant)	Number of plants tested	Observed GFP segregation ratio (positive:negative)	1:1	3:1	15:1
T₁ plants
Mn-1	13	8:5	0.69^a	1.26^a	23.02
Mn-2	179	125:47	35.37	0.50^a	130.39
Mn-4	Sterile plant
Mn-7	30	19:11	2.13^a	2.18^a	47.37
T₂ plants
Mn-1(1)	5	3:2	0.20^a	0.6^a	9.72
Mn-1(2)	6	6:0	Homozygous plants
Mn-2(3)	15	15:0	Homozygous plants
Mn-2(4)	47	36:11	13.30	0.06^a	23.60
Mn-2(7)	34	24:10	5.77	0.35^a	31.13
Mn-2(10)	18	18:0	Homozygous plants
Mn-7(2)	12	6:6	0.00^a	4.00	39.2
Mn-7(3)	33	16:17	0.03^a	12.38	115.40
Mn-7(4)	18	10:8	0.22^a	3.63^a	44.82

^a if χ² value is above 3.84 (P > 0.05) the observed segregation ratio is not significantly different from the expected ratio

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