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Table 2 Impact of medium composition on genetic transformation frequency of domesticated einkorn T.monococcum L.

From: Genetic transformation of einkorn (Triticum monococcum L. ssp. monococcum L.), a diploid cultivated wheat species

Induction medium Selection medium No. of bombarded explants No. of GFP positive morphogenic structures survived the selection No. of regenerated plantlets that survived the selection Number of independent transgenic plants (PCR+) Transformation efficiency (%)
Basala Ac 265 3 2 1 0.38
Bd 271 2 1 0 0.00
Total/Average 534 5 3 1 0.19
Modifiedb Ac 245 8 2 1 0.41
Bd 295 7 2 2 0.68
Total/Average 540 15 4 3 0.56
  1. aMS basal salts and vitamins, 150 mg/l L-asparagine, 3 mg/l Dicamba, 50 mg/l Daminozide, 0.25 mg/l TDZ
  2. bMS basal salts and vitamins, 150 mg/l L-asparagine, 3 mg/l Dicamba, 50 mg/l Daminozide, 0.1 mg/l TDZ
  3. cMS basal salts and vitamins, 150 mg/l L-asparagine, 3 mg/l Dicamba, 25 mg/l Daminozide, 3 mg/l PPT
  4. dMS basal salts and vitamins, 150 mg/l L-asparagine, 3 mg/l Dicamba, 3 mg/l PPT