Genetic transformation of einkorn (Triticum monococcum L. ssp. monococcum L.), a diploid cultivated wheat species

Table 2 Impact of medium composition on genetic transformation frequency of domesticated einkorn T.monococcum L.

Induction medium	Selection medium	No. of bombarded explants	No. of GFP positive morphogenic structures survived the selection	No. of regenerated plantlets that survived the selection	Number of independent transgenic plants (PCR+)	Transformation efficiency (%)
Basal^a	A^c	265	3	2	1	0.38
	B^d	271	2	1	0	0.00
	Total/Average	534	5	3	1	0.19
Modified^b	A^c	245	8	2	1	0.41
	B^d	295	7	2	2	0.68
	Total/Average	540	15	4	3	0.56

^aMS basal salts and vitamins, 150 mg/l L-asparagine, 3 mg/l Dicamba, 50 mg/l Daminozide, 0.25 mg/l TDZ
^bMS basal salts and vitamins, 150 mg/l L-asparagine, 3 mg/l Dicamba, 50 mg/l Daminozide, 0.1 mg/l TDZ
^cMS basal salts and vitamins, 150 mg/l L-asparagine, 3 mg/l Dicamba, 25 mg/l Daminozide, 3 mg/l PPT
^dMS basal salts and vitamins, 150 mg/l L-asparagine, 3 mg/l Dicamba, 3 mg/l PPT

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