Skip to main content

Table 1 Composition of culture media for domesticated einkorn (T.monococcum L.) tissue culturea

From: Genetic transformation of einkorn (Triticum monococcum L. ssp. monococcum L.), a diploid cultivated wheat species

Media (procedure) Phytohormones, mg/l Carbohydrates, % PPT, mg/l Duration period
Dicamba Daminozide TDZ GA3 Sucrose Mannitol
Callus induction medium basal 3 50 0.25   3    5–15 days
Callus induction medium modified 3 50 0.1   3   
Osmoticum medium 3 50    3 7.3   20 hours
Callus selection medium A 3 25    3   3 6 weeks
Callus selection medium B 3     3   3
Callus proliferation medium 1.5     3   3 3 weeks
Differentiation medium 0.5     3   3 2–3 weeks
Regeneration medium      3   3 3–5 weeks
Rooting medium      2   2 2–4 weeks
Embryo germination medium     0.1 2    5–10 days
  1. aMS medium consisted of MS basal salts and vitamins supplemented with 150 mg/l L-asparagine and 7 g/l agar was used as basic medium in all cases