Table 1 Composition of culture media for domesticated einkorn (T.monococcum L.) tissue culturea
Media (procedure) | Phytohormones, mg/l | Carbohydrates, % | PPT, mg/l | Duration period | ||||
|---|---|---|---|---|---|---|---|---|
Dicamba | Daminozide | TDZ | GA3 | Sucrose | Mannitol | |||
Callus induction medium basal | 3 | 50 | 0.25 |  | 3 |  |  | 5–15 days |
Callus induction medium modified | 3 | 50 | 0.1 | Â | 3 | Â | Â | |
Osmoticum medium | 3 | 50 | Â | Â | 3 | 7.3 | Â | 20Â hours |
Callus selection medium A | 3 | 25 | Â | Â | 3 | Â | 3 | 6Â weeks |
Callus selection medium B | 3 | Â | Â | Â | 3 | Â | 3 | |
Callus proliferation medium | 1.5 | Â | Â | Â | 3 | Â | 3 | 3Â weeks |
Differentiation medium | 0.5 |  |  |  | 3 |  | 3 | 2–3 weeks |
Regeneration medium |  |  |  |  | 3 |  | 3 | 3–5 weeks |
Rooting medium |  |  |  |  | 2 |  | 2 | 2–4 weeks |
Embryo germination medium |  |  |  | 0.1 | 2 |  |  | 5–10 days |