Fig. 3

Molecular analyses of putative transgenic einkorn plants. a Schematic diagrams of psGFP-BAR vector used for einkorn transformation. Thick bar represents the positions of bar probe. b, c Primary plants produced within experiment were analyzed for the presence of bar gene by PCR amplification (b) and GFP expression by RT-PCR(c); Lane M, DNA ladder as molecular weight marker; Lane P, psGFP-BAR; Lane C, untransformed einkorn plant; Lane 1–6 represents putative transgenic plants Mn-1, Mn-2, Mn-4, Mn-5, Mn-6, Mn-7, respectively; +, a sample with addition of reverse transcriptase; −, a same sample without addition of reverse transcriptase. d Southern blot analyses of genomic DNAs from PCR positive primary transgenic plants; genomic as well as plasmid control DNA was digested with HindIII and the membrane was probed using the 310-bp bar probe; Lane P, psGFP-BAR (1.0 ng); Lane Cneg, negative control representing DNA from untransformed einkorn plant; Cpos, positive control, consisting of DNA from transgenic bread wheat plant with two transgene insertions; Lines Mn-1, Mn-2, Mn-4, Mn-7, primary transgenic einkorn plants