Fig. 2

Selection of transgenic einkorn tissues after biolistic-mediated transformation with psGFP-BAR. a, b Transient GFP expression in morphogenic explant, 24 h after bombardment. c, d Formation of GFP-positive transgenic embryo, 55 days of culture. e, f Formation of chimerical (transgenic/untransgenic) cluster, 65 days of culture. g, h Development of transgenic regenerating plant, 75 days of culture. Tissues were photographed under white light (upper panel) or blue light (lower panel) using the GFP filter set (EX BP 470/40, BS FT 495, EM LP 550)