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Table 1 Central composite design matrix, experimental and predicted values for response surface analysis

From: Bioconversion of duck blood cell: process optimization of hydrolytic conditions and peptide hydrolysate characterization

Run Independent variables Response
Coded value Actual value DH (%)
x 1 x 2 x 3 x 4 T1 (°C) C2 (%) pH Time (h) Observed Predicted
1 −1 1 1 −1 45 18 8.0 6.0 25.45 25.42
2 −1 1 1 1 45 18 8.0 7.0 26.46 26.99
3 1 1 −1 −1 55 18 7.0 6.0 28.77 28.58
4 2 0 0 0 60 16 7.5 6.5 23.64 24.14
5 0 2 0 0 50 20 7.5 6.5 30.66 30.77
6 0 0 0 0 50 16 7.5 6.5 31.26 31.91
7 0 0 0 0 50 16 7.5 6.5 31.46 31.91
8 1 −1 −1 −1 55 14 7.0 6.0 28.14 27.73
9 1 -1 -1 1 55 14 7.0 7.0 30.55 30.83
10 1 -1 1 1 55 14 8.0 7.0 31.68 31.54
11 0 0 0 0 50 16 7.5 6.5 32.16 31.91
12 −2 0 0 0 40 16 7.5 6.5 19.13 18.27
13 −1 −1 1 −1 45 14 8.0 6.0 25.82 26.15
14 1 1 −1 1 55 18 7.0 7.0 31.17 30.96
15 0 0 0 0 50 16 7.5 6.5 31.81 31.91
16 0 0 0 0 50 16 7.5 6.5 32.56 31.91
17 1 −1 1 −1 55 14 8.0 6.0 28.30 28.56
18 −1 1 −1 −1 45 18 7.0 6.0 25.54 25.80
19 −1 1 −1 1 45 18 7.0 7.0 27.50 27.49
20 0 0 −2 0 50 16 6.5 6.5 29.79 29.83
21 0 0 0 0 50 16 7.5 6.5 32.38 31.91
22 1 1 1 −1 55 18 8.0 6.0 28.97 28.86
23 0 0 0 2 50 16 7.5 7.5 33.61 33.27
24 −1 −1 −1 1 45 14 7.0 7.0 28.15 28.38
25 0 0 2 0 50 16 8.5 6.5 30.57 30.16
26 0 −2 0 0 50 12 7.5 6.5 31.84 31.36
27 0 0 0 −2 50 16 7.5 5.5 28.63 28.60
28 1 1 1 1 55 18 8.0 7.0 31.21 31.12
29 −1 −1 1 1 45 14 8.0 7.0 28.00 28.44
30 −1 −1 −1 −1 45 14 7.0 6.0 25.64 25.98
  1. 1Temperature
  2. 2substrate concentration
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