Fig. 5

Expression of CeA–Mxe–ELK16 fusion in the SA-ELK16 system and its optimization. Cells producing the CeA–Mxe–ELK16 fusion were cultured at 16 °C for 24 h. Different concentrations of IPTG (0.2–1.0 mM) were tested to maximize expression. Positions corresponding to the CeA–Mxe–ELK16 fusion are marked with red arrows. NO, the cells were not induced with IPTG; W, whole-cell lysate; S, supernatant fractions; and P, insoluble pellets of induced cells