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Fig. 5 | BMC Biotechnology

Fig. 5

From: CRISPR/Cas9-mediated genome editing induces gene knockdown by altering the pre-mRNA splicing in mice

Fig. 5

Knockdown Ccnb3 expression does not have an effect on spermatogenesis and male fertility. a Ccnb3 protein in adult wild type and Ccnb3 mutant mouse testes, detected by Western blot. b Adult wild type and Ccnb3 mutant mouse testes and epididymis. c Testis weight of adult wild type and Ccnb3 mutant mice. d H&E staining of adult testis and epididymis of wild type and Ccnb3 mutant. a and a’ were the cross section of testes; b and b’ were the cross section of epididymides. ST: seminiferous tubule; Sg: spermatogonia; Sc: spermatocytes; Sz: spermatozoa; ET: epididymal tubule. Arrows showed the Sg, Sc or Sz, respectively. Double-headed arrow showed the location of spermatogenic cells, which contains Sg, Sc and Sz. e Sperm count of adult wild type and Ccnb3 mutant mice. f Litter size of adult wild type and Ccnb3 mutant male mice. Bar = 100 μm

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