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Fig. 3 | BMC Biotechnology

Fig. 3

From: CRISPR/Cas9-mediated genome editing induces gene knockdown by altering the pre-mRNA splicing in mice

Fig. 3

Knock down Ccnb3 expression by deletion its exon 1/intron 1 boundary using CRISPR/Cas9. a RT-PCR of Ccnb3 shows the expression of Ccnb3 in wild type (+/O) and mutant (−/O) mouse testes. b Ccnb3-/O mutant mouse’s Ccnb3 mRNA splicing. c Wild type mouse’s Ccnb3 mRNA splicing. d Wild type and four splicing mutation of Ccnb3’s part of amino acid sequence. .: stop codon. Red letter show the difference of amino acid sequence between wild type and splicing mutation 1 of Ccnb3

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