Table 4 Comparative effects of site-directed mutagenesis and saturation mutagenesis
From: Consensus design for improved thermostability of lipoxygenase from Anabaena sp. PCC 7120
Enzyme | Probability (%) | Half-life (t1/250°C) (min) |
|---|---|---|
N130D | 55 | 10.2 ± 0.4 |
N130S | 11 | 7.2 ± 0.3 |
G260E | 55 | 4.7 ± 0.3 |
G260A | 0 | 9.2 ± 0.4 |
S437R | 55 | < 2.3 ± 0.1 |
S437T | 0 | 9.3 ± 0.5 |
S437K | 0 | 6.4 ± 0.4 |