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Table 4 Comparative effects of site-directed mutagenesis and saturation mutagenesis

From: Consensus design for improved thermostability of lipoxygenase from Anabaena sp. PCC 7120

Enzyme Probability (%) Half-life (t1/250°C) (min)
N130D 55 10.2 ± 0.4
N130S 11 7.2 ± 0.3
G260E 55 4.7 ± 0.3
G260A 0 9.2 ± 0.4
S437R 55 < 2.3 ± 0.1
S437T 0 9.3 ± 0.5
S437K 0 6.4 ± 0.4