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Table 2 Characterization of mutations

From: Consensus design for improved thermostability of lipoxygenase from Anabaena sp. PCC 7120

Enzyme Specific activity (IU/mg) Km (μmol/L) kcat (1/s) kcat/Km
L/(mmol·s)
WT 10.4 ± 0.2 56 ± 4.0 30.3 ± 1.6 541.1
G260A 22.5 ± 0.8 34 ± 2.3 51.7 ± 2.5 1520.6
S437T 27.6 ± 0.8 56 ± 4.7 117.6 ± 6.4 2100.0
N130D 22.7 ± 0.5 30 ± 2.6 57.7 ± 1.8 1923.3
N130D/S437Y 15.2 ± 0.9 15 ± 1.1 31.8 ± 1.9 2120.0
N130D/G260Q 21.0 ± 0.6 13 ± 1.0 35.4 ± 2.6 2724.6