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Table 2 Characterization of mutations

From: Consensus design for improved thermostability of lipoxygenase from Anabaena sp. PCC 7120

Enzyme

Specific activity (IU/mg)

Km (μmol/L)

kcat (1/s)

kcat/Km

L/(mmol·s)

WT

10.4 ± 0.2

56 ± 4.0

30.3 ± 1.6

541.1

G260A

22.5 ± 0.8

34 ± 2.3

51.7 ± 2.5

1520.6

S437T

27.6 ± 0.8

56 ± 4.7

117.6 ± 6.4

2100.0

N130D

22.7 ± 0.5

30 ± 2.6

57.7 ± 1.8

1923.3

N130D/S437Y

15.2 ± 0.9

15 ± 1.1

31.8 ± 1.9

2120.0

N130D/G260Q

21.0 ± 0.6

13 ± 1.0

35.4 ± 2.6

2724.6