Table 1 Thermal stability of mutants
From: Consensus design for improved thermostability of lipoxygenase from Anabaena sp. PCC 7120
Enzyme | Optimal temperature (°C) | Half-life(t1/250°C) (min) | Tm (°C) |
|---|---|---|---|
WT | 35 | 2.3 ± 0.1 | 48.17 |
G260A | 35 | 9.2 ± 0.4 | 48.61 |
S437T | 45 | 9.3 ± 0.5 | 49.42 |
N130D | 40 | 10.2 ± 0.4 | 49.59 |
N130D/S437Y | 45 | 11.0 ± 0.5 | 50.28 |
N130D/G260Q | 40 | 15.2 ± 0.9 | 50.62 |