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Fig. 3 | BMC Biotechnology

Fig. 3

From: Development of a vitrification method for preserving human myoblast cell sheets for myocardial regeneration therapy

Fig. 3

Effect of vitrification on mitochondrial function and structure. a Evaluation of mitochondrial structure in the fresh and vitrification groups by TEM. Scale bar, 1 μm. b Summary of RT-PCR results for the expression levels of the mitochondria-associated genes mt-ND1 and mt-ATP6 in the fresh and vitrification groups. The fresh group was evaluated before cryopreservation. The cryopreserved cell-sheet constructs of the vitrification group were thawed at 2 days (D), 1 week (W), 1 month (M), and 3 months for assessment. The results are expressed as the means ± SD (n = 8 independent experiments); *p < 0.01

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BMC Biotechnology

ISSN: 1472-6750

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