Fig. 3

Disruption of 3′ splice site at the boundary of intron 3 and exon 4 of locust LmigOr35 causes complete exon 4 skipping. a RT-PCR shows that 156 bp nucleotides are missed in the 55 bp mutant (−/−) locusts compared with the WT (+/+) locusts. b WT locusts (+/+) RT-PCR product sequencing shows that exons 3 and 4 of locust LmigOr35 normally combine with each other. c 55 bp mutant locusts (−/−) RT-PCR product sequencing shows that exon 3 of locust LmigOr35 combine with exon 5 of locust LmigOr35 and exon 4 is skipped. d The WT locust LmigOr35 pre-mRNA splicing. Exons 3, 4, and 5 of locust LmigOr35 normally join with each other after splicing. e The 55 bp mutant locust LmigOr35 pre-mRNA splicing. Exons 3 and 5 of locust LmigOr35 combine with each other and exon 4 is skipped after the splicing