Fig. 1

Disrupting the 3′ splice site by deletion of 55 bp nucleotides at the boundary of intron 3 and exon 4 of locust LmigOr35 using CRISPR/Cas9 system. a The entire gene structure of LmigOr35 with all introns and exons and the designed sgRNA targeted site in exon 4 of locust LmigOr35. F1 and R1 are primers for detecting genome deletion; F2 and R2 are primers for detecting exon deletion. b Deleted nucleotides containing 15 bp intron 3 and 40 bp exon 4 nucleotides of locust LmigOr35. Under line shows the conserved nucleotides in the splice site that are crucial for normal splicing. c Genotype of WT (+/+) and 55 bp mutant (−/−) locusts. The WT locusts obtain a 485 bp brand and the 55 bp mutants obtain a 430 bp brand