Fig. 8From: A rapid in vitro method to flip back the double-floxed inverted open reading frame in a plasmidProtocol overview. The input plasmid is first mixed with Cre enzyme in 1× reaction buffer. After 20–30 min of incubation at 37 °C, the reaction is brought to 70 °C for 10 min to heat inactivate Cre recombinase. The whole reaction can then be directly used for transformationBack to article page