In vitro recombination assays: Time course and Cre enzyme dose effect. a-b Time course. a Example gel image. DNA was digested by XhoI BsrGI. DNA bands for different products are marked. b Time course plot illustrating the change in recombination products over the time. The results are averaged from three independent experiments. Error bars mean S.E.M. c-d Effects of increasing Cre on recombination. c Example gel image. d Plot of recombination products as function on cre concentrations. The results are averaged from three independent experiments. Error bars mean S.E.M.