Fig. 5
From: A rapid in vitro method to flip back the double-floxed inverted open reading frame in a plasmid
In vitro recombination assays: Effects of DIO sizes. a Example gel image of in vitro recombination assay. Three plasmids of different DIO sizes were examined. Each was digested with different restriction enzyme combinations in order to distinguish different recombination products. Plasmid # 3 (DIO 0.9) was digested with XhoI and BsrG1. Plasmid # 4 (DIO 1.5) was digested with BamHI, XhoI and BstXI. Plasmid # 1 (DIO 2.4) was digested with XhoI, BsrGI and NsiI. DNA bands for different products are marked. 1, 2, 3, nr represent R1, R2, R3, non-Recombination respectively. b Bar charts showing the percentage of R1 (B1), R3 (B2) and RT (B3). (B4) Comparisons of R2 (left) and R1/R2 ratios (right) between plasmids #3 (0.9 kb DIO) and plasmids # 4 (1.5 kb DIO). The results are averaged from three independent experiments. Bars indicate the S.E.M. #, p < 0.05, ##, p < 0.01, one way ANOVA; *, p < 0.05, **, p < 0.01, Tukey multi-comparison test