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Fig. 4 | BMC Biotechnology

Fig. 4

From: A rapid in vitro method to flip back the double-floxed inverted open reading frame in a plasmid

Fig. 4

Effects of DIO sizes and concentrations of Cre on cloning efficiencies. a Effects of DIO sizes. Bar charts showing the percentage of R1 colonies (A1), R3 colonies (A2) and total recombinants (RT) (A3). The results are averaged from three independent experiments. Bars indicate the S.E.M. #, p < 0.05, one way ANOVA; *, p < 0.05, Tukey multi-comparison test. b Effects of Cre concentrations. Bar charts showing the percentage of R1 colonies (B1), R3 colonies (B2) and RT (B3). The results are averaged from three independent experiments. Bars indicate the S.E.M

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