Fig. 3
From: A rapid in vitro method to flip back the double-floxed inverted open reading frame in a plasmid
Higher numbers of two-step recombination was obtained from a plasmid with shorter DIO sequence. a Diagram of input plasmid. b NheI/EcoRI digestion. Seven plasmids (#1,3,5,7,9,10,11) have flipped ORF as they showed band of 772 bp. These plasmids could be either R1 or R3 according to predicted patterns (right panel). c BamhI/EcoRI digestion. Bands of 998 bp and 830 bp were yielded from R1 (#1,3,7,10) and R3 (#5,9,11), respectively. Right panel, predicted patterns. d-e Example images of HEK cells transfected with plasmid # 2 (NR) (d) and #1(R1) (e). As expected, fluorescence was detected in e, but not in d