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Fig. 2 | BMC Biotechnology

Fig. 2

From: A rapid in vitro method to flip back the double-floxed inverted open reading frame in a plasmid

Fig. 2

Cre recombinase effectively inverted ORF in FLEX plasmid in vitro. a Inversion of ORF in plasmid #1. Top, plasmid diagram. (A1) PstI screening. A 926 bp band by pst1 digestion of R1 can be seen form 5 plasmids (# 2,3,7,8,12). There were no R2 and R3 plasmids (should yield a 758 bp band). Colony #1 had low yield. (A2) Plasmid Map. Shown are five PstI sites and two NcoI sites. (A3) Prediction of PstI digestion. b Inversion of ORF in plasmid #2. Top, plasmid diagram. (B1) NcoI digestion. An 813 bp band by NcoI digestion of R1 can be seen from 4 plasmids (# 5,6,7,8). #11 could be either R2 or R3 plasmids for the presence of 645 bp band. (B2) Plasmid Map. PstI and NcoI sites are shown. (B3) Prediction of NcoI digestion

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