Fig. 1From: A rapid in vitro method to flip back the double-floxed inverted open reading frame in a plasmidScenarios of in vitro flip of ORF in FLEX plasmid. a Cre-mediated recombination. Starting from input plasmid, Cre enzyme can induce ORF inversion via two LoxP sites (R1) or two Lox2722 sites (R2). Excising linker sequence between two Lox sites of the same orientation in either R1 or R2 yields R3. Purple triangles, LoxP sites; Blue triangles, Lox2272 sites. b Diagram of Cre-mediated reactions at equilibriumBack to article page