Proof of concept in utilizing in-trans surface display system of Lactobacillus plantarum as mucosal tuberculosis vaccine via oral administration in mice

Table 3 Primers used for amplification of the ACER and LysM genes

Genes	Sequence 5′ ➔ 3’^a	Optimal Tm (°C)
ACER	Fwd: GGATCCGCTGACCAGCGAGCTGCCGCAA	54
ACER	Rev: CTGCAGTGGCTTCCCTTCCGAAACCGC	54
ACE	Fwd:GGATCCGCTGACCAGCGAGCTGCCGCAA	56
ACE	Rev:CTGCAGGATATCAGATCTCGCGTTGTTCAGCTCGGTAGC	56
R0R2	Fwd:GGATCCGAACCTGCGTGAGCGTGCGGAG	54
R0R2	Rev: CTGCAGTGGCTTCCCTTCCGAAACCGC	54
LysM	Fwd: CTGCAGGGTACCACTACTTATACCGTCAAATC	54
LysM	Rev: GCGGCCGCTTATTTTATTCGTAGATACTG	54

^aRestriction enzyme (RE) sites are shown as bold and underlined as either BamHI (GGATCCG), PstI (CTGCAG) or NotI (GCGGCCGC)

ISSN: 1472-6750