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Table 2 Bacterial strains and plasmids used/or developed in this study

From: Proof of concept in utilizing in-trans surface display system of Lactobacillus plantarum as mucosal tuberculosis vaccine via oral administration in mice

Strain/Plasmid Characteristics Source
Strains
L. lactis NZ9000 MG1363 pepN::nisRK, host strain for all lactococcal plasmids [44]
E. coli Rossetta (DE3) pLysS Expression host for all E. coli vectors Novagen
Plasmids
 pRSF:Duet-1 ~ 3.8 kb, KanR, T7 promoter Invitrogen
 pNZ8048 ~ 3.3 kb, CamR [44]
 pJet:ACE ~ 3.6 kb, ApR, pJET1.2 vector derivatives containing fusion M. tuberculosis antigen of Ag85B, CFP10 and ESAT6 (ACE) with corresponding RE sites for sub-cloning purpose IDT
 pJet:R0R2 ~ 3.3 kb, ApR, pJET1.2 vector derivatives containing fusion M. tuberculosis antigen of Rv0475 and Rv2031c (R0R2) with corresponding RE sites for sub-cloning purpose IDT
 pNZ:mIL-12 ~ 5.0 kb, CmR, pNZ8048 vector derivatives containing mouse IL-12 gene downstream of PnisA Gift from JK,IBS, UPM
 pRSF:LysM ~ 5.6 kb, KanR, pRSFDuet-1 vector derivatives containing LysM anchor motif with corresponding RE sites for sub-cloning purpose downstream of T7 promoter This study
 pRSF:ACERL ~ 5.6 kb, KanR, pRSFDuet-1 vector derivatives containing fusion M. tuberculosis antigen gene of Ag85B, CFP10, ESAT6 Rv0475, Rv2031c and LysM (ACERL) with corresponding RE sites for sub-cloning purpose downstream of T7 promoter This study