Table 2 Bacterial strains and plasmids used/or developed in this study

 L. lactis NZ9000

MG1363 pepN::nisRK, host strain for all lactococcal plasmids

[44]

 E. coli Rossetta (DE3) pLysS

Expression host for all E. coli vectors

Novagen

 pRSF:Duet-1

~ 3.8 kb, Kan^R, T7 promoter

Invitrogen

 pNZ8048

~ 3.3 kb, Cam^R

[44]

 pJet:ACE

~ 3.6 kb, Ap^R, pJET1.2 vector derivatives containing fusion M. tuberculosis antigen of Ag85B, CFP10 and ESAT6 (ACE) with corresponding RE sites for sub-cloning purpose

IDT

 pJet:R0R2

~ 3.3 kb, Ap^R, pJET1.2 vector derivatives containing fusion M. tuberculosis antigen of Rv0475 and Rv2031c (R0R2) with corresponding RE sites for sub-cloning purpose

IDT

 pNZ:mIL-12

~ 5.0 kb, Cm^R, pNZ8048 vector derivatives containing mouse IL-12 gene downstream of PnisA

Gift from JK,IBS, UPM

 pRSF:LysM

~ 5.6 kb, Kan^R, pRSFDuet-1 vector derivatives containing LysM anchor motif with corresponding RE sites for sub-cloning purpose downstream of T7 promoter

This study

 pRSF:ACERL

~ 5.6 kb, Kan^R, pRSFDuet-1 vector derivatives containing fusion M. tuberculosis antigen gene of Ag85B, CFP10, ESAT6 Rv0475, Rv2031c and LysM (ACERL) with corresponding RE sites for sub-cloning purpose downstream of T7 promoter

This study