Fig. 4

Immunofluorescence microscopy of L. plantarum interacted with ACERL protein (a) and 1× PBS as negative control (b). (i) FITC conjugated secondary antibody staining for L. plantarum interacted with ACERL protein or 1× PBS while (ii) is phase contrast outcome for L. plantarum interacted with ACERL protein (a) or 1× PBS (b). The positive FITC signalling by L. plantarum cells interacted with ACERL protein indicated successful docking of the target protein to live L. plantarum cell wall. Note that L. plantarum displayed ACERL was probed with mouse anti-his primary antibody and stained with anti-mouse IgY secondary antibody conjugated with FITC. (Images were observed under: × 100 oil immersion objective)