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Fig. 2 | BMC Biotechnology

Fig. 2

From: Proof of concept in utilizing in-trans surface display system of Lactobacillus plantarum as mucosal tuberculosis vaccine via oral administration in mice

Fig. 2

Detection of ACERL produced from E. coli. a Over-expression of recombinant fusion proteins of ACERL expressed from E. coli Rossetta (DE3) host via SDS-PAGE analysis. Cells were induced with 1 mM/ml IPTG, agitated for 6 h at 30 °C.Label 1–3 indicates the protein of interest ACERL expression profiles whereLane M: PageRuler™ PrestainedPlus Protein Ladder (Fermentas, Canada); Lane 1: Uninduced ACERL; Lanes 2: Induced ACERL from the intracellular fraction; Lanes 3: Induced ACERL from the lysate pellet fraction. ACERL was observed in the lysate pellet fraction only as indicated by the arrow. b SDS-PAGE of soluble fraction of ACERL treated with 5% NLS. Lane m: PageRuler™ Unstained Protein Ladder (Fermentas, Canada); Lane 1: ACERL intracellular fraction; Soluble recombinant ACERL proteins was achieved and detected at ~ 64 kDa respectively as indicated by arrow

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BMC Biotechnology

ISSN: 1472-6750

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